SHRI SHIVAJI SCIENCE COLLEGE, AMRAVATI
DBT STAR COLLEGE PROJECT ACTIVITY
ACTIVITY REPORT
Workshop on Plant Tissue Culture
Activity Dates: 24.09.2025
Type of Activity: Students Workshop
Organizing Department: Department of Microbiology
Program Coordinators: Prof. Komal D. Nagpure, Prof. Shravani N. Kalkar
Head of the Department: Dr. R. C. Maggirwar
External Collaborator (if any): -
Objectives:
- 1. To Gain Hands-on Experience in Tissue Culture Techniques
- 2. To Explore the Applications of Plant Tissue Culture in Agriculture and Biotechnology:
- 3. To Learn Advanced Plant Biotechnology Technique
- 4. To Encourage Research and Career Pathways in Plant Biotechnology
- 5. To Understand the Fundamentals of Plant Tissue Culture
No of Beneficieries: 40
Classes Involved: B.Sc-III Year Microbiology & Biotechnology students
Venue of the Activity: Department of Microbiology, Shri Shivaji Science College, Amravati
Activity Report:
A Workshop on Plant Tissue Culture for B.Sc. III Year Biotechnology students was conducted on 24th September 2025, offering an in-depth exploration of plant biotechnology techniques, with a special focus on plant tissue culture applications. The workshop aimed to equip students with essential theoretical knowledge and hands-on experience in the field of plant tissue culture, a critical technique used in agriculture, conservation, and biotechnology. The workshop was organized to help students understand the process of plant cloning, genetic modification, and the propagation of endangered plants.
The event featured a key guest speaker, Dr. C. M. Wadatkar, a renowned expert from Shivaji Agriculture College, Amravati. Dr. Wadatkar shared his extensive knowledge on the importance of plant tissue culture in crop improvement and sustainable agriculture, highlighting its applications in micro propagation and the development of disease-resistant plants. His talk emphasized the future prospects of plant biotechnology and its role in solving global challenges, such as food security and environmental conservation.
The workshop included theoretical sessions on the basic principles of plant tissue culture, explaining the process of media preparation, hormonal regulation, and the creation of culture environments. The practical session allowed students to gain hands-on experience in the sterilization of explants, media preparation, and the transfer of plant tissues to culture vessels. Additionally, advanced techniques like somatic embryogenesis and genetic transformation were discussed, expanding students' understanding of the cutting-edge applications in plant biotechnology.
By the end of the workshop, students had gained practical insights into plant tissue culture, enhancing their technical skills and motivating them to pursue further studies or careers in plant biotechnology. The workshop successfully provided a platform for learning and discussion, fostering an interest in this vital area of biotechnology.
Introduction:
On 24th September 2025, a one-day workshop on Plant Tissue Culture was conducted for the B.Sc. III Year Biotechnology students. The workshop aimed to provide students with a comprehensive understanding of the principles and techniques of plant tissue culture, an important biotechnological tool used in plant research and agriculture. Plant tissue culture is widely used for cloning plants, developing genetically modified crops, and preserving endangered plant species. This hands-on workshop was designed to introduce students to the various stages of the tissue culture process, including the sterilization of explants, preparation of culture media, and the growth of plantlets under controlled conditions.
The workshop was led by experts in plant biotechnology, who guided the students through theoretical aspects as well as practical demonstrations. This session not only aimed to enhance students' technical skills but also to spark their interest in the application of tissue culture in sustainable agriculture, conservation, and plant biotechnology.
Overview:
The workshop began at 10:00 AM with a welcome address by the workshop coordinator, followed by an introduction to plant tissue culture. The students were briefed on its importance in the field of biotechnology and its various applications, such as micro propagation, genetic modification, and the development of disease-resistant plants.
Key sessions included:
1. Theoretical Session (10:30 AM - 12:00 PM):
o An overview of plant tissue culture techniques was provided, including the history of tissue culture, basic principles, and types of tissue culture such as callus culture, meristem culture, and organogenesis.
o Discussion on culture media preparation, types of hormones (auxins and cytokinins), and their role in plant growth.
2. Practical Demonstration (12:00 PM - 1:30 PM):
o Students participated in hands-on activities, such as preparing culture media, sterilizing explants, and transferring plant tissues to the culture vessels.
o The focus was on aseptic techniques and maintaining sterile conditions to prevent contamination during tissue culture procedures.
3. Lunch Break (1:30 PM - 2:30 PM):
o A break for lunch was provided, offering an opportunity for students and faculty to interact and discuss the techniques learned during the morning session.
4. Advanced Techniques (2:30 PM - 3:30 PM):
o A session on advanced techniques in plant tissue culture, including genetic transformation, somatic embryogenesis, and the cryopreservation of plant cells, was held.
o This session aimed to expand students' understanding of biotechnology applications in agriculture and conservation.
5. Q&A and Discussion (3:30 PM - 4:00 PM):
o The workshop concluded with a Q&A session, where students interacted with experts, clarified doubts, and discussed the potential applications of plant tissue culture in research and industry.
Outcomes:
The workshop was successful in achieving several key outcomes for the participating students:
1. Understanding the Basics of Plant Tissue Culture:
Students gained a solid understanding of the theoretical principles and practical techniques involved in plant tissue culture. They learned about the critical steps such as explant preparation, sterilization, and media formulation.
2. Hands-on Practical Experience:
Students actively engaged in practical activities, giving them direct experience with preparing culture media and handling plant tissues under sterile conditions. This exposure is essential for their understanding of lab-based biotechnological processes.
3. Exposure to Advanced Techniques:
The session on advanced techniques, such as somatic embryogenesis and genetic transformation, broadened students’ knowledge of current advancements in plant biotechnology.
4. Increased Interest in Biotechnology:
The workshop sparked curiosity among students, motivating them to explore plant tissue culture as a career option or research area. They were introduced to real-world applications such as crop improvement, bioremediation, and plant conservation.
5. Collaboration and Networking:
Students had the opportunity to interact with experts and peers, discussing potential research opportunities in the field of plant biotechnology.
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Conclusion:
The Plant Tissue Culture Workshop on 24th September 2025 proved to be a highly informative and enriching experience for the B.Sc. III Year Biotechnology students. By combining theoretical knowledge with hands-on practice, the workshop successfully demonstrated the importance of plant tissue culture in modern biotechnology and agriculture. Students gained valuable insights into the application of tissue culture in plant breeding, genetic engineering, and sustainable agriculture.
This workshop not only enhanced their technical skills but also encouraged them to consider further research in plant biotechnology. The knowledge gained will be beneficial for their future academic pursuits and professional careers in biotechnology, especially in fields such as agriculture, horticulture, and conservation biology.
Overall, the workshop was a great success, achieving its goal of providing students with a practical, engaging introduction to plant tissue culture and its applications in biotechnology.
Outcomes:
- 1. Understanding the Basics of Plant Tissue Culture
- 2. Hands-on Practical Experience
- 3. Exposure to Advanced Techniques
- 4. Increased Interest in Biotechnology
- 5. Collaboration and Networking
Photos:
 A great start to our PTC workshop |  Beginning the journey of hands on learning at the PTC workshop |
 A moment of meaningful interaction |  Enthusiastic participation from our students at PTC training session |
 Presentation of the Detailed protocols for PTC workshop |  A successful and enriching PTC workshop with dedicated students |
Attendance Sheet:
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